LOCUS       p4RZ-CGFP    7722 bp    DNA   circular             2-NOV-2005
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|272044412|
COMMENT     VNTDBDATE|390042540|
COMMENT     VNTNAME|p4RZ-CGFP|
COMMENT     VNTAUTHORNAME|Kevin Morris|
COMMENT     VNTOAUTHORNAME|David J. Looney, M.D.|
COMMENT     VNTOAUTHORTEL|858-552-8585x2626|
COMMENT     VNTOAUTHORFAX|858-52-7416|
COMMENT     VNTOAUTHOREML|dlooney@ucsd.edu|
COMMENT     VNTOAUTHORAD1|Dept. of Medicine 0678|
COMMENT     VNTOAUTHORAD2|University of California San Diego|
COMMENT     VNTOAUTHORAD3|Stein Clinical Research Bldg Room 302|
COMMENT     VNTOAUTHORAD4|La Jolla, CA 92093-0678 USA|
FEATURES             Location/Qualifiers
     LTR             complement(3997..5470)
                     /vntifkey="19"
                     /label=5'LTR
                     /note="HIV-2 5'LTR"
     LTR             complement(7570..1303)
                     /vntifkey="19"
                     /label=3'LTR
                     /note="HIV-2 3'LTR"
     CDS             complement(1306..2097)
                     /vntifkey="4"
                     /label=eGFP
                     /note="clonetechs eGFP from pEGFP-C1"
     misc_feature    3032..3987
                     /vntifkey="21"
                     /label=4RZ\ribozyme\cassette
                     /note="4RZ ribozyme cassette"
     loci            2710..3031
                     /vntifkey="18"
                     /label=RRE
                     /note="HIV RRE"
     misc_feature    2118..2698
                     /vntifkey="21"
                     /label=pCMV
                     /note="CMV promoter"
BASE COUNT     1783 a      2011 c      1889 g      2035 t         4 others
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acgataggcc 
      421 taacaccgaa tgaccaggcg gcgactagga gagatgggaa cacacactta acttgcttct 
      481 aactggcagc tttattaaga ggtctttaag caagcaagcg tggagccgtc tgcccagtac 
      541 cggccaagta ctggtgagag tctagcaggg aacacccagg ctctacctgc tagtgctgga 
      601 gagaacctcc cagggctcga tctgccagcc tctccgcaga gcgactgaat acaatgcaag 
      661 cagcgggtac atttatacag aagtatgagg gcgttcccca ccggttcctc ccacgtccct 
      721 cccctggtta cagccccttc tggaaagtcc ctgcagtttc atcatctact tcctgttctg 
      781 accaaatata gctgttcctg tctttaacta aatggtatcc ctcttgcttt cagttttgcc 
      841 ttccactctt cttctggcag gcctgactta tacccaaatt cttctgggta tcgattaaaa 
      901 gccgtatact cataggccag cctagggtca aacctccaca ttaatgtttc cccatgaggg 
      961 tcatcagacc cgcttgtttg tgctgggtgt agtaagcagt ggtcctcttc accttgtggg 
     1021 acgtctactg gtactagctt ccatagccac ccaaagaact ttgggtacct tactcctggc 
     1081 ccatgagtat agttctgcca atctggaatt atcccttctt ccttttccat gtatatgtct 
     1141 aagattctat gtccttctct cactgtaata catcccatcc aggtcccccc tttttcattt 
     1201 agaaaatgtg atatatctac tgccaactta tatgtcattg cccttaatgg tactcttggt 
     1261 gtgacaggga cccctattag gttatcatta tctgcatcta catcatccag ttggggatcc 
     1321 cgggcccgcg gtaccgtcga ctgcagaatt cgaagcttga gctcgagatc tgagtccgga 
     1381 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac 
     1441 catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc tcaggtagtg 
     1501 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc 
     1561 gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt 
     1621 cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg 
     1681 ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag 
     1741 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa 
     1801 gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt 
     1861 catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg tcacgagggt 
     1921 gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta 
     1981 ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc 
     2041 cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatggt 
     2101 ggcgaccggt agcgctagcg gatctgacgg ttcactaaac cagctctgct tatatagacc 
     2161 tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt 
     2221 ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga 
     2281 cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc 
     2341 atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat 
     2401 aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg 
     2461 ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac 
     2521 tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt 
     2581 attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt 
     2641 tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat 
     2701 taataactat tccaatcagg tactatgctg ttattttccc atagtacagt ggtgtagcag 
     2761 acttgtctaa atgcacatcc ccatgaattt agccgcgcct ggtcccttag gtatttctcg 
     2821 atagcagtga ctcttgtctg gagatttttt gttccccaga cggtcagtcg caacatttct 
     2881 tgttgtctct tgaccacgtc caacagctgt tgctgttgct gcactatccc agcccagtaa 
     2941 agtccgggat gagccgacag cgtcaaggac gccgcgccca ttgcagaacc tgctgttgcg 
     3001 agaaaaccca agactcctag cacaaacaca ttctagatgc tctctaagcc ggcgagcgat 
     3061 cgcaattcag gactagtctt ttaggtcaaa aagaagaagc tttgtaaccg ttggtttccg 
     3121 tagtgtagtg gttatcacgt tcgcctcaca cgcgaacggt ccccggttcg aaaccgggcg 
     3181 gaaacagggc cccgaggcgc atgcttgtat gcaagaacca aaccagagaa acacacggac 
     3241 ttcggtccgt ggtatattac ctggtatttt tgcatttttg cggccgcaat tcaggactag 
     3301 tcttttaggt caaaaagaag aagctttgta accgttggtt tccgtagtgt agtggttatc 
     3361 acgttcgcct cacacgcgaa cggtccccgg ttcgaaaccg ggcggaaaca cctaggcggg 
     3421 tagacgtcgt atgtcattag aagtccacca gagaaacaca cgttgtggta tattacctgg 
     3481 tatttttgca tttttggcgc caattcagga ctagtctttt aggtcaaaaa gaagaagctt 
     3541 tgtaaccgtt ggtttccgta gtgtagtggt tatcacgttc gcctcacacg cgaacggtcc 
     3601 ccggttcgaa accgggcgga aacattaatt aacggcgcac cggtttatgt tagaaggtga 
     3661 ccagagaaac acacggactt cggtccgtgg tatattaatt caggactagt cttttaggtc 
     3721 aaaaagaaga agctttgtaa ccgttggttt ccgtagtgta gtggttatca cgttcgcctc 
     3781 acacgcgaac ggtccccggt tcgaaaccgg gcggaaacat taattaacgg cgcaattgaa 
     3841 aaanaaattn ganccacnga atgagagaga gatctgacac aacaagaagg caaccagaga 
     3901 aacacacgtt gtggtatatt acctggtacg cgtggacgta agtccttttt gcatttttcc 
     3961 aaaaaatggt ctagactcga gtctagacta gagctagagt cgagccggcg gtgctgctgg 
     4021 tctacttgtg cttggcattt tgtctgcagt tcctgtttct gccactagat gtctctgtac 
     4081 tatttgtttt gctccttcag tatctttcac tttctcttct gcgtgcaagc accaaatgac 
     4141 gcagacagta ttaaaaaggc tttttaaatt ttctgatcct gttggcacta atggatctaa 
     4201 aactgtaata attttttggc aaccttcttt tgactccaac aggctttctg ccaatccgaa 
     4261 tttgcccaat tcattcgctg cccacacaat atgctttagt ctatattttt tcttcccgcc 
     4321 gggccgtaac ctaatttttt ctaattcatc tacttttttc cctctcaaga cggagcttct 
     4381 cgcgcccatc tcccacaatc ttctacctgt ctaaaggtag gataacaagc ctttttctgg 
     4441 ctacagtttt tggtgtaggt acttaccttc acccggaggc ctctttactc ccgctccaca 
     4501 cgccgccttt ggtacctcgg cccgcgcctt tctaggagca ctccgtcgtg gtttgttcct 
     4561 gccgccctta ctgccttcac tcagccgtgt tccagtctcc ttcaagtcct gttcgggcgc 
     4621 caatctgcta ggtattttcc cgcttcggtt ccccaaagca agaagggtcc taacagacca 
     4681 gggtcttgtc acttaggtga acaccgaatg accaggcggc gactaggaga gatgggaaca 
     4741 cacacttaac ttgcttctaa ctggcagctt tattaagagg tctttaagca agcaagcgtg 
     4801 gagccgtctg cccagtaccg gccaagtact ggtgagagtc tagcagggaa cacccaggct 
     4861 ctacctgcta gtgctggaga gaacctccca gggctcgatc tgccagcctc tccgcagagc 
     4921 gactgaatac aatgcaagca gcgggtacat ttatacagaa gtatgagggc gttccccacc 
     4981 ggttcctccc acgtccctcc cctggttaca gccccttctg gaaagtccct gcagtttcat 
     5041 catctacttc ctgttctgac caaatatagc tgttcctgtc tttaactaaa tggtatccct 
     5101 cttgctttca gttttgcctt ccactcttct tctggcaggc ctgacttata cccaaattct 
     5161 tctgggtatc gattaaaagc cgtatactca taggccagcc tagggtcaaa cctccacatt 
     5221 aatgtttccc catgagggtc atcagacccg cttgtttgtg ctgggtgtag taagcagtgg 
     5281 tcctcttcac cttgtgggac gtctactggt actagcttcc atagccaccc aaagaacttt 
     5341 gggtacctta ctcctggccc atgagtatag ttctgccaat ctggaattat cccttcttcc 
     5401 ttttccatgt atatgtctaa gattctatgt ccttctctca ctgtaataca tcccatccaa 
     5461 cgcgttcgac ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt 
     5521 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 
     5581 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 
     5641 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 
     5701 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 
     5761 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 
     5821 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 
     5881 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 
     5941 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 
     6001 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 
     6061 ccgcctttct cccttcggga agcgtggcgc tttctcaaag ctcacgctgt aggtatctca 
     6121 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 
     6181 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 
     6241 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 
     6301 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 
     6361 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 
     6421 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 
     6481 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 
     6541 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 
     6601 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 
     6661 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 
     6721 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 
     6781 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 
     6841 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 
     6901 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 
     6961 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 
     7021 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 
     7081 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 
     7141 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 
     7201 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 
     7261 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 
     7321 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 
     7381 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 
     7441 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 
     7501 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 
     7561 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 
     7621 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga 
     7681 cattaaccta taaaaatagg cgtatcacga ggccctttcg tc 
//