Position: Graduate Student

Nationality: USA

Education: : BS

Email: yevgeniyg@scripps.edu

MicroRNAs (miRNAs), discovered in 1993, are evolutionarily conserved, small noncoding, gene-silencing, 22-nt long RNAs found in Eukaryotes. Animal miRNAs regulate gene expression at the level of translation by using imperfect target complementarity with cognate mRNA in the 3' untranslated regions (UTRs), resulting in translational repression and/or degradation of the target transcript. Regulatory roles of miRNAs have been implicated in diverse developmental, physiological and pathogenic events. However, the role and function of miRNAs in regulating human T and B lymphocyte activation in models for acute, antigen-driven immune responses has not been studied. We are interested in studying the role that differentially expressed miRNAs play in regulating human T and B lymphocyte activation and if they do so by targeting highly differentially expressed and/or alternative spliced genes. Our study encompasses a systematic genome-wide analysis of alternative splicing as a function of activation in human lymphocytes while also inclusively analyzing genome-wide differential gene and miRNA expression.

PUBLICATIONS:

Deconvoluting post-transplant immunity: cell subset-specific mapping reveals pathways for activation and expansion of memory T, monocytes and B cells. Grigoryev YA, Kurian SM, Avnur Z, Borie D, Deng J, Campbell D, Sung J, Nikolcheva T, Quinn A, Schulman H, Peng SL, Schaffer R, Fisher J, Mondala T, Head S, Flechner SM, Kantor AB, Marsh C, Salomon DR.PLoS One. 2010 Oct 14;5(10):e13358.

Genome-wide analysis of immune activation in human T and B cells reveals distinct classes of alternatively spliced genes.Grigoryev YA, Kurian SM, Nakorchevskiy AA, Burke JP, Campbell D, Head SR, Deng J, Kantor AB, Yates JR 3rd, Salomon DR.PLoS One. 2009 Nov 19;4(11):e7906.

Applying genomics to organ transplantation medicine in both discovery and validation of biomarkers.Kurian S, Grigoryev Y, Head S, Campbell D, Mondala T, Salomon DR. Int Immunopharmacol. 2007 Dec 20;7(14):1948-60. Epub 2007 Aug 9. Review.