Characteristics of Three NADH Dehydrogenases in Bacteria
NDH-1 NDH-2 Na+-NDH
Ion-pump H+ (and Na+?) not a pump Na+
Cofactors one noncovalent FMN
2 [2Fe-2S]
6 - 8 [4Fe-4S]		 one noncovalent FAD one noncovalent FAD
two covalent FMN
1 [2Fe-2S]a
Subunit Composition 13-14 1 6
Inhibitors Rotenone
Piericidin A
Capsaicin
Acetogenins
Pyridaben
Fenpyroximate		 Flavone (?) Ag+
HQNO
Korormicin
Organisms Paracoccus denitrificans
Rhodobacter capsulatus
Escherichia coli
Synechocystis PCC6803
Thermus thermophilus
Klebsiella pneumoniae		 many gram-positive bacteria
many archaea
Vibrio
Haemophilus influenzae
Escherichia coli>/td> 		Vibrio
Haemophilus influenzae
Pseudomonas aeruginosa
Klebsiella pneumoniae
a According to a recent paper by Steuber et al. (2002), the [2Fe-2S] cluster assigned to Na+-NDH in Vibrio alginolyticus (Pfenninger-Li et al., 1996) appears to belong to the succinate dehydrogenase or the fumarate reductase.