Screening the mammalian extracellular proteome for regulators of embryonic human stem cell pluripotency. Proc Natl Acad Sci U S A. 2010 Feb 2. Gonzalez R, Jennings LL, Knuth M, Orth AP, Klock HE, Ou W, Feuerhelm J, Hull MV, Koesema E, Wang Y, Zhang J, Wu C, Cho CY, Su AI, Batalov S, Chen H, Johnson K, Laffitte B, Nguyen DG, Snyder EY, Schultz PG, Harris JL, Lesley SA.

Crystal Structure of the First Eubacterial Mre11 Nuclease Reveals Novel Features that may Discriminate Substrates During DNA Repair. J Mol Biol. 2010 Jan 29. Das D, Moiani D, Axelrod HL, Miller MD, McMullan D, Jin KK, Abdubek P, Astakhova T, Burra P, Carlton D, Chiu HJ, Clayton T, Deller MC, Duan L, Ernst D, Feuerhelm J, Grant JC, Grzechnik A, Grzechnik SK, Han GW, Jaroszewski L, Klock HE, Knuth MW, Kozbial P, Krishna SS, Kumar A, Marciano D, Morse AT, Nigoghossian E, Okach L, Paulsen J, Reyes R, Rife CL, Sefcovic N, Tien HJ, Trame CB, van den Bedem H, Weekes D, Xu Q, Hodgson KO, Wooley J, Elsliger MA, Deacon AM, Godzik A, Lesley SA, Tainer JA, Wilson IA.

Bacterial pleckstrin homology domains: a prokaryotic origin for the PH domain. J Mol Biol. 2010 Feb 12;396(1):31-46. Xu Q, Bateman A, Finn RD, Abdubek P, Astakhova T, Axelrod HL, Bakolitsa C, Carlton D, Chen C, Chiu HJ, Chiu M, Clayton T, Das D, Deller MC, Duan L, Ellrott K, Ernst D, Farr CL, Feuerhelm J, Grant JC, Grzechnik A, Han GW, Jaroszewski L, Jin KK, Klock HE, Knuth MW, Kozbial P, Krishna SS, Kumar A, Marciano D, McMullan D, Miller MD, Morse AT, Nigoghossian E, Nopakun A, Okach L, Puckett C, Reyes R, Rife CL, Sefcovic N, Tien HJ, Trame CB, van den Bedem H, Weekes D, Wooten T, Hodgson KO, Wooley J, Elsliger MA, Deacon AM, Godzik A, Lesley SA, Wilson IA.

Parallel methods for expression and purification. Methods Enzymol. 2009;463:767-85. Lesley SA.

Three-dimensional structural view of the central metabolic network of Thermotoga maritima. Science. 2009 Sep 18;325(5947):1544-9. Zhang Y, Thiele I, Weekes D, Li Z, Jaroszewski L, Ginalski K, Deacon AM, Wooley J, Lesley SA, Wilson IA, Palsson B, Osterman A, Godzik A.

Oxidase activity of a flavin-dependent thymidylate synthase. FEBS J. 2009 May;276(10):2801-10. Wang Z, Chernyshev A, Koehn EM, Manuel TD, Lesley SA, Kohen A.

An unusual mechanism of thymidylate biosynthesis in organisms containing the thyX gene. Nature. 2009 Apr 16;458(7240):919-23. Koehn EM, Fleischmann T, Conrad JA, Palfey BA, Lesley SA, Mathews II, Kohen A.

The Thermotoga maritima Trk potassium transporter--from frameshift to function. J Bacteriol. 2009 Apr;191(7):2276-84. Johnson HA, Hampton E, Lesley SA.

The Polymerase Incomplete Primer Extension (PIPE) method applied to high-throughput cloning and site-directed mutagenesis. Methods Mol Biol. 2009;498:91-103. Klock HE, Lesley SA.

Structure and ligand binding of the soluble domain of a Thermotoga maritima membrane protein of unknown function TM1634. Protein Sci. 2008 May;17(5):869-77. McCleverty CJ, Columbus L, Kreusch A, Lesley SA.

Protein production and purification. Nat Methods. 2008 Feb;5(2):135-46 Gräslund S, Nordlund P, Weigelt J, Hallberg BM, Bray J, Gileadi O, Knapp S, Oppermann U, Arrowsmith C, Hui R, Ming J, dhe-Paganon S, Park HW, Savchenko A, Yee A, Edwards A, Vincentelli R, Cambillau C, Kim R, Kim SH, Rao Z, Shi Y, Terwilliger TC, Kim CY, Hung LW, Waldo GS, Peleg Y, Albeck S, Unger T, Dym O, Prilusky J, Sussman JL, Stevens RC, Lesley SA, Wilson IA, Joachimiak A, Collart F, Dementieva I, Donnelly MI, Eschenfeldt WH, Kim Y, Stols L, Wu R, Zhou M, Burley SK, Emtage JS, Sauder JM, Thompson D, Bain K, Luz J, Gheyi T, Zhang F, Atwell S, Almo SC, Bonanno JB, Fiser A, Swaminathan S, Studier FW, Chance MR, Sali A, Acton TB, Xiao R, Zhao L, Ma LC, Hunt JF, Tong L, Cunningham K, Inouye M, Anderson S, Janjua H, Shastry R, Ho CK, Wang D, Wang H, Jiang M, Montelione GT, Stuart DI, Owens RJ, Daenke S, Schütz A, Heinemann U, Yokoyama S, Büssow K, Gunsalus KC.

Selecting folded proteins from a library of secondary structural elements. J Am Chem Soc. 2008 Jan 9;130(1):176-85 Graziano JJ, Liu W, Perera R, Geierstanger BH, Lesley SA, Schultz PG.

Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts. Proteins. 2008 May 1;71(2):982-94. Klock HE, Koesema EJ, Knuth MW, Lesley SA.

The self-inhibited structure of full-length PCSK9 at 1.9 A reveals structural homology with resistin within the C-terminal domain. Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14604-9. Hampton EN, Knuth MW, Li J, Harris JL, Lesley SA, Spraggon G.

The relationships between oxidase and synthase activities of flavin dependent thymidylate synthase (FDTS). Chem Commun (Camb). 2007 Jul 19;(27):2861-3. Chernyshev A, Fleischmann T, Koehn EM, Lesley SA, Kohen A.

Crystal structure of the human TRPV2 channel ankyrin repeat domain. Protein Sci. 2006 Sep;15(9):2201-6. McCleverty CJ, Koesema E, Patapoutian A, Lesley SA, Kreusch A.

Crystal structure of a divalent metal ion transporter CorA at 2.9 angstrom resolution. Science. 2006 Jul 21;313(5785):354-7. Eshaghi S, Niegowski D, Kohl A, Martinez Molina D, Lesley SA, Nordlund P.

Expression, purification, and characterization of Thermotoga maritima membrane proteins for structure determination. Protein Sci. 2006 May;15(5):961-75. Authors: Columbus L, Lipfert J, Klock H, Millett I, Doniach S, Lesley SA.

High-throughput protein production for X-ray crystallography and use of size exclusion chromatography to validate or refute computational biological unit predictions. J Struct Funct Genomics. 2005;6(2-3):135-41. McMullan D, Canaves JM, Quijano K, Abdubek P, Nigoghossian E, Haugen J, Klock HE, Vincent J, Hale J, Paulsen J, Lesley SA.

Methods and results for semi-automated cloning using integrated robotics. J Struct Funct Genomics. 2005;6(2-3):89-94. Klock HE, White A, Koesema E, Lesley SA.

On the use of DXMS to produce more crystallizable proteins: structures of the T. maritima proteins TM0160 and TM1171. Protein Sci. 2004 Dec;13(12):3187-99. Spraggon G, Pantazatos D, Klock HE, Wilson IA, Woods VL, Lesley SA.

A scaleable and integrated crystallization pipeline applied to mining the Thermotoga maritima proteome. J Struct Funct Genomics. 2004;5(1-2):133-46. DiDonato M, Deacon AM, Klock HE, McMullan D, Lesley SA.

Rapid refinement of crystallographic protein construct definition employing enhanced hydrogen/deuterium exchange MS. Proc Natl Acad Sci U S A. 2004 Jan 20;101(3):751-6. Pantazatos D, Kim JS, Klock HE, Stevens RC, Wilson IA, Lesley SA, Woods VL.

Structural genomics of the Thermotoga maritima proteome implemented in a high-throughput structure determination pipeline. Proc Natl Acad Sci U S A. 2002 Sep 3;99(18):11664-9. Lesley SA, Kuhn P, Godzik A, Deacon AM, Mathews I, Kreusch A, Spraggon G, Klock HE, McMullan D, Shin T, Vincent J, Robb A, Brinen LS, Miller MD, McPhillips TM, Miller MA, Scheibe D, Canaves JM, Guda C, Jaroszewski L, Selby TL, Elsliger MA, Wooley J, Taylor SS, Hodgson KO, Wilson IA, Schultz PG, Stevens RC.