Analysis of actin dynamics at the cell edge of Ptk1 kidney epithelial cells using fluorescent speckle microscopy (FSM).  The presence of active RacQ61L causes a widening of the lamellipodia and increased retrograde actin flow.  This effect is blocked when the downstream effector, p21-activated kinase 1 (PAK1) is inhibited by expressing of the (auto)inhibitory domain (PID).  See Delorme et al (2007) – in press.
(Movies prepared by Violaine Delorme, Ph.D)

Normal HeLa cell undergoing cell division.  See Birkenfeld et al Dev. Cell.  May 8, 2007. Vol 12, Issue 5, pages  699-712.
(Movie prepared by Joerg Birkenfeld, Ph.D.)

Movie 6: 

Cells undergoing division:  note the cell to the left fails to divide properly due to the enhanced cofilin activity caused by overexrpression of the  cofilin phosphatase Chronophin (CIN), discovered in our laboratory.  See Gohla et al Nature Cell Biol. 7: 21, 2005.
(Movie prepared by Céline DerMardirossian, Ph.D.)

Movie S1:

A clonal HeLa cell ine stably expressing Cofilin1-GFP treated with Azide/2-deoxyglucose to deplete cellular ATP for 30 minutes. Cofilin-GFP is aggregated into large tapered rod inclusions by 30 minutes of ATP-depletion.
(Movie prepared by Timothy Huang, Ph.D.)

Movie S3:

Cofilin1-GFP cells transfected with a CIN-targeting shRNA construct (simultaneously expressing mRFP, left field) are subjected to ATP depletion, and the rate of Cofilin1-GFP rod formation is compared between shRNA-expressing and non-expressing cells. CIN shRNA experssing reduces the rate of Cofilin1-GFP rod formation during ATP depletion.
(Movie prepared by Timothy Huang, Ph.D.)

Movie S4:

Cofilin1-GFP cells have been ATP-depleted for 30 minutes, and subjected to glucose repletion in normal growth media for 30 minutes. Cofilin1-GFP images are acquired immediately after glucose repletion, and Cofilin1-GFP can be seen to rapidly disperse from rod-shaped inclusions to the cell periphery.
(Movie prepared by Timothy Huang, PhD.)