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Submitting Samples for Analysis
RNA Submission
Samples will not be processed until a sample submission form has been received, and an experiment release form has been signed by the submitting Investigator. To submit samples, contact Brandon Young. You will be given a login ID and password for our LIMS site via e-mail. This site will allow you to provide information on each sample, choose an assay type, track progress and download your data upon completion of your project.
Samples submitted to the facility should be in the form of total RNA. The Genomics Core highly recommends following the RNA Isolation SOP RNA01. Total RNA samples can be brought to the west end of RF-1 Rm 113. Please do not leave your samples unattended without speaking to facility staff member. We recommend calling 6-8830 before bringing your samples to room 113 to make sure someone is available to receive them. All RNAs will be checked for quality on the BioRad Experion or Agilent Bioanalyzer system before processing for expression.
Requirements for submitted total RNAs are:
Affymetrix
- RNA concentration must be greater than or equal to 200ng/μl.
- Minimum of 2μg of total RNA is required for each GeneChip.
- Tubes must be clearly labeled with the same name indicated on the submission form, as well as the RNA concentration.
Illumina
- RNA concentration must be greater than or equal to 100ng/μl.
- Minimum of 0.5μg of total RNA is required for each experiment.
- Tubes must be clearly labeled with the same name indicated on the submission form, as well as the RNA concentration.
The Investigator will be notified if RNA samples have low concentrations. Low RNA samples such from sources such as single cell, LCM, or small tissues can be processed using a two-cycle amplification. To request a two-cycle amplification experiment or alternative vendor kit, please arrange a meeting with the facility advisor. To set up a meeting contact Brandon Young. Two cycle amplification samples may incur additional cost and may take longer than two weeks to complete. Estimates on cost and timeline will be made after meeting with the facility advisor.
DNA Submission
Samples will not be processed until a sample submission form has been received, and an experiment release form has been signed by the submitting Investigator. To submit samples, contact Brandon Young. You will be given a login ID and password for our LIMS site via e-mail. This site will allow you to provide information on each sample, choose an assay type, track progress and download your data upon completion of your project.
RNA samples can be brought to the west end of RF-1 Rm 113. Please do not leave your samples unattended without speaking to facility staff member. We recommend calling 6-8830 before bringing your samples to room 113 to make sure someone is available to receive them. All RNAs will be checked for quality on an agarose gel before processing.
Requirements for submitted total DNAs are:
Affymetrix
- DNA concentration must be greater than or equal to 100ng/μl.
- Minimum of 1μg of total RNA is required for each GeneChip.
- Tubes must be clearly labeled with the same name indicated on the submission form, as well as the RNA concentration.
Illumina
- DNA concentration must be greater than or equal to 100ng/μl.
- Minimum of 1 μg of total DNA is required for each experiment.
- Tubes must be clearly labeled with the same name indicated on the submission form, as well as the RNA concentration.
Sequenom
- DNA concentration must be greater than or equal to 10ng/μl.
- Minimum of 0.2μg of total DNA is required.
- DNAs should be submitted in 96-well format, with sample ID map in an excel sheet.
- Actual parameters may differ slightly; hence a consultation is required at the beginning of the project. Contact Brandon Young (byoung@scripps.edu).
The Investigator will be notified if DNA samples have low concentrations.
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