Scripps Florida Logo

Genomics Core

Welcome to the Genomics Core at The Scripps Research Institute (TSRI)-Florida!

Our goal is to provide Scripps Florida investigators as well as external investigators to the latest technologies for Next Generation Sequencing (NGS) and microarray analysis. 

We strive to provide the best service possible to our research community and encourage all investigators interested in incorporating sequencing analysis to their projects to contact us.  The Genomics Core owns the latest NGS platforms to address a wide variety of research needs. Consultation on sample preparation and procedures is also provided and highly recommended before starting your projects.  Research can also be performed on a collaborative basis with the Genomics Core staff for protocol optimization to meet each investigator’s demands. 

The Genomics Core maintains and operates the following platforms: 

  • SOLID5500XL (Life Technologies)
  • Ion Proton and Ion Personal Genome Machine (PGM) (Ion Torrent)
  • NextSeq 500 (Illumina) 
  • Affymetrix GeneChip

 We also provide the following equipment: 

  • Ion Chef system (Ion template preparation, chip loading, and Ampli-seq library prep for Ion Torrent platforms)
  • NeoPrep (digital microfluidic library prep system) for Illumina platforms
  • Sonicators (Bioruptor and S2 Covaris)
  • Real time PCR machines (Step One plus and Light Cycler 480)
  • Agilent 2100 Bioanalyzer
  • Agilent 2200 TapeStation

 These technologies allow the core to support a wide array of experimental approaches including: 

  • Whole gene expression profiling
  • whole transcriptome sequencing (polyA enrichment or rRNA-depletion)
  • small RNA sequencing
  • Ultra-low input and single-cell RNA sequencing
  • Regulatory DNA or RNA interactions with proteins (RIP-seq, ChIP-seq) analysis
  • Splice variant quantification
  • Copy number variation
  • Secondary validation of array data
  • Whole genome association studies

For more information, please contact:

Pabalu Karunadharma, Ph.D.

B206/B244b

Ph: (561) 228-2493

pkarunad@scripps.edu