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Genomics

Frequently Asked Questions

  1. Does the core provide RNA or DNA extraction services?
  2. What are the sample quality guidelines?
  3. Does the Genomics Core run quality assurance checks on submitted samples?
  4. How are samples submitted?
  5. How long until I get my results?
  6. How long do you store experimental data?
  7. What are the costs for each experiment?
  8. Do you accept projects from outside institutions?
  9. What are the costs for each experiment?
  10. Do you accept projects from outside institutions?
  1. Does the core provide RNA or DNA extraction services?

    No, individual investigators are responsible for extracting nucleic acids.

  2. What are the sample quality guidelines?

    In order to ensure the most accurate results all samples should be checked for concentration, A260/280 as well as gel electrophoresis prior to submission.

  3. Does the Genomics Core run quality assurance checks on submitted samples?

    Yes. The Genomics Core staff will check all submitted samples by running samples on Agilent 2100 Bioanalyzer.  If any sample fails QC at this point, the investigator will be notified. The investigator will then have the option to continue with the experiment or submit new samples. The core cannot make any guarantees for the quality of data that is generated using samples that do not pass initial QC checkpoints.

  4. How are samples submitted?

    All samples should be in either nuclease-free water or low-EDTA TE buffer. It is the responsibility of the researcher to provide samples in a buffer that is compatible with library preparation. Two forms should be submitted to the core.  Both forms can be found here.  As a reminder, the Genomics Core will not accept samples without prior submission of both forms.  The Principal Investigator signature agreeing to pay the cost of the experiment and account number information for billing should be included in one of the forms.  

  5. How long until I get my results?

    Typical turnaround time is around 2 weeks from time of receipt of samples and sample submission form.  However, this will depend on the total number of samples provided by the investigator and other samples ready to be run.  The Genomics Core will try to run a particular fixed number of samples per sequencing platform to reduce costs for each investigator.  Investigators will be notified once the quality of their samples is assessed for a more accurate timing. 

  6. Do you perform data analysis?

    The core will perform preliminary data analysis. If further analysis is required, please contact Gustavo Martinez for discussion.

  7. How long do you store submitted samples?

    Samples will be kept until the experiment is completed, and the investigator confirms that no further data is required using the same samples.  At this point, the remainder of the samples will be returned to the investigator.

  8. How long do you store experimental data?

    Analyzed data will be accessible to the investigator for one month.  The investigator is responsible for downloading the analyzed data into their own servers.

    Raw data will be stored by the Genomics Core share, and stored for a year.  The investigator is responsible for downloading the raw data into their own server once this time expiries.  Notifications will be sent once month priori to the deletion of either raw or analyzed data.

  9. What are the costs for each experiment?

    For pricing, please check out the Genomics Core rates here For large scale projects or specific discounts, contact Gustavo Martinez.

  10. Do you accept projects from outside institutions?

    Yes, the Genomics Core will provide services for institutions other than Scripps Florida. The external rates for each service provided can be found here.  For specific projects or questions, please contact Gustavo Martinez.