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Genomics - Affymetrix Microarray

The Genomics Core houses a complete Affymetrix GeneChip system including a hybridization oven, an automated fluidics station allowing parallel processing of 8 chips and a high-resolution scanner. Affymetrix GeneChips whole genome expression chips are designed to provide a complete profile of gene expression in a given sample. GeneChips can be used to interrogate differences stemming from drug treatment, gene knock-down (using siRNA or shRNA), gene knockouts and natural biological effects, among others.

Services Offered

Full Chip Service: Bring your total RNA (10ug total which has been Trizol isolated and then RNAeasy (Qiagen) purified. The facility then performs quality analysis of total RNA, cRNA synthesis, labeling, hybridization, scanning, and data extraction.
Hybridization and Scanning Only: Bring your cRNA probe labeled with biotin to the facility. The facility will add the proper control oligos and cRNA, hybridize, wash, scan and extract raw data. The users assume all responsibility for failed hybridizations. Turn around time is usually 1 week. As soon as the results are ready, the investigator will be contacted via email.

Gene chips :

Most chips will require additional time for ordering.

We encourage all investigators to briefly discuss their experiment with Pabalu Karunadharma prior to submission of samples.  RNA or DNA samples are directly submitted to the Genomics Core with the appropriate forms. The quality/quantity of the received nucleic acids will be evaluated using Bioanalyzer.  The staff will also prepare the samples and run the hybridization and scanning.  A preliminary bioinformatics analysis will be performed.