Services & Contact Information

Sorting on the BD FACSAriaII

 The FACSAriaII is a three-laser system configuration. The FACSAriaII uses a 488nm (blue), 633nm (red), and a 407nm (violet) laser system.

 Sorting using up to thirteen fluorescence parameters as well as light scatter discrimination (cell size, organelle composition and density, and doublet discrimination). Applications include high speed, four-way cell sorting, cell purification, slide sorting, cell cloning (by automated deposition into 6, 12, 24, 48, 96 well plates), and sorting of potentially hazardous materials (human samples, retro-virally transduced cells) consistent with BSL2 containment.

These services will be performed solely by facility staff.

Analysis on the BD LSRII

    The BD LSRII is a five-laser configuration. The LSRII uses a 488nm (blue), 561nm (yellow green), 640nm (red), 407 nm (violet), and a 355nm (UV) laser system. Analyses of up to fourteen fluorescence parameters as well as light scatter discrimination (cell size, organelle composition and density, and doublet discrimination). Initial applications will include immunophenotyping, cell cycle analysis (DNA content), and calcium flux (ratiometric imaging). Other modalities (FRET, intracellular organelle staining, etc.) will be developed in response to need.

    These services will be provided on two levels:

1. Authorized users- after training by facility staff, with additional application development and/or troubleshooting as required.
2. Operation by facility staff- this service would only be provided in instances where
   1. Facility staff is required for operation of the LSRII.
   2. For infrequent users, in which case it is not useful to learn the software and hardware principles.

Laser Capture Microdissection on the Leica AS LMD microscope

    The Leica LMD laser capture microdissection microscope has 4X, 6.3X, 10X, 20X, 40X, and 63x objectives, automated motorized scanning and positioning, and remote stylus operated input.

    Laser capture microdissection allows for the precise and contamination-free isolation of specific areas of tissue (e.g. tumor material) from single cells or cell groups according to morphological criteria. The dissected material is then directly accessible for further analysis.

Hemavet 950 FS

    The Hemavet 950 FS is an animal blood cell counter/differential analyzer. The Hemavet allows for a fast and complete five-part WBC differential. It includes 20 parameters including platelets. The Hemavet is fully automated and simple to operate. Whole blood analysis is available for mouse and rat blood specimens with as little as 30ul. Tissue culture counting is also available.