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Flow Cytometry

Flow Cytometry Core Notice To all: We are pleased to welcome Alta Johnson to the staff. Alta comes to use from The University of Florida at Gainesville, where she had some experience in analytical cytometry, but not with cell sorting. Alta started two weeks ago today, and we believe she has made exceptional progress in learning how to operate and maintain facility equipment. In addition, Bivian Torres very kindly agreed to delay the onset of her transition to Miami, and a 3 day per week commute to Scripps, until the week after her wedding (10 February), enabling more hands-on time for getting Alta up to speed as quickly as possible.Beginning on 12 February, and until we believe that Alta is ready to handle cell sorting on her own, sorting will be limited to Tuesday, Wednesday, and Thursday of each week (Bivian’s days on-site). If there is an urgent need for sorting on a Monday or Friday, please contact us; it may be possible for Bivian to alter her schedule and/or, for less complicated procedures, Alta may be sufficiently prepared to take them on alone (more so as time goes on). Bivian is also scheduled to be out on 26 and 29 January, as well as 8 an 9 February, so there will likewise be no sorting on those dates. We anticipate that the Facility should be back to normal within another month or two, at most. While we transition as quickly as possibly to full functionality, please don’t hesitate to contact any one of us, and we will do our best to accommodate your needs if we can. Thanks for your patience.

Normal operating hours: 9:00am-6:00pm, M-F

End-user access available 24/7 after training

Flow cytometry measures phenotypic, biochemical and molecular characteristics of individual cells or particles suspended in a fluid stream. The cells flow single file past focused laser beams and light scatter or fluorescence data is collected. Tens of thousands of cells can be interrogated by a flow cytometer in a single second. Flow cytometry can be used for a variety of applications, most commonly including cell analysis or high speed cell sorting. The Scripps Florida Flow Cytometry Core (FCC) serves the Scripps Florida community as well as researchers from outside Scripps on a fee-for-service basis.

Technologies available:

  • Cell Sorting
    • ARIA: violet, blue, green, and red lasers
    • FUSION: violet, blue, green, and red lasers
  • Cell Analysis
    • LSR2: ultra-violet, violet, blue, green and red lasers
    • GALLIOS: violet, blue, green, and red lasers
    • CANTO: violet, blue and red lasers
  • Laser Microdissection 
    • LEICA LMD 7000
  • Hematology
    • HEMAVET 950FS

Contact Info and Key Personnel:

To learn more about the core and to inquire about services and scheduling please send email to facility staff at flowcore@scripps.edu or call at 561-228-3293.  If you have any concerns that you feel are not being addressed, suggestions for improvement, or new technologies that you would like to develop, please feel free to contact any one of us in person or at the email addresses above.

Location: 130 Scripps Way C276, Jupiter Fl, 33458