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Analytical Cytometry

Analytical applications include immuno-phenotyping, cell cycle analysis (DNA content, BrdU), and calcium flux (ratiometric imaging), among others. Additional modalities (FRET, intracellular organelle staining, etc.) can be developed in response to need; please talk to us about your specific application.

The Core provides three different analyzers for use: a 5 laser BD LSR2, a 4 laser Beckman Coulter Gallios, and a 2 laser BD Canto. Facility staff can assist users in deciding which analyzer is best for their given applications.  Cost recovery is based on laser configuration (more lasers = higher cost; see Pricing).

Services are provided on two levels:

  1. End-user analysis: training is available and is encouraged for routine users. Facility staff must authorize each new user, even if they have previously trained at other institutions.
  2. Operation by facility staff: for infrequent users, it may not be useful to learn the software and hardware principles.  Consequently, facility staff may provide assistance (at standard charge-back rates) for these users, based on staff availability.

All independent users are expected to follow all startup/shutdown procedures as well as Core Facility rules.

BD LSR2

The BD LSR2 is a five-laser configuration (UV 355nm, violet 407nm, blue 488nm, yellow-green 561 nm, and red 640nm lasers). Analysis of up to eighteen fluorescence parameters is possible, as well as visible light scatter discrimination for cell size, organelle composition or density, and doublet discrimination. The LSR2 also features a High Throughout Sampler (HTS) for a fully automated sample acquisition. The HTS is compatible with 96-and 384-well plate formats.

LSR2 Instrument Configuration and Standard Fluorochromes

Beckman Coulter Gallios

The Beckman Coulter Gallios is a four-laser configuration (violet 405nm, blue 488nm, yellow-green 561 nm, and red 638nm lasers). Analysis of up to ten fluorescence parameters is possible, as well as visible light scatter discrimination for cell size, organelle composition or density, and doublet discrimination. The Gallios also features a 32-tube carousel loader that allows for automated tube sampling and analysis.

Gallios Instrument Configuration and Standard Fluorochromes

BD Canto

The BD Canto is a three-laser configuration (violet 405nm, blue 488nm and red 640nm lasers). Analysis of up to eight fluorescence parameters is possible, as well as visible light scatter discrimination for cell size, organelle composition or density, and doublet discrimination.

Canto Instrument Configuration and Standard Fluorochromes