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Flow Cytometry
Flow Cytometry
Flow cytometry measures and analyzes the characteristics of single particles, normally cells, as they move in a stream and are passed through a laser. Thousands of cells can be analyzed by a flow cytometer in a single second. Among the measurements derived from flow cytometry are the size, relative fluorescence and complexity of the particle. The Flow Cytometry group uses two state-of-the-art multiple laser cell sorting, purification, and cloning flow systems (the Becton-Dickinson FACSAria and LSRII) to perform analyses on up to fourteen fluorescence parameters as well as light scatter discrimination experiments (cell size, organelle composition and density, doublet discrimination). In addition, a Leica LMD laser dissection microscope is available for the precise and contamination-free isolation of specific areas of tissue (e.g. tumor material) from single cells or cell groups according to morphological criteria.
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