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Contact & Information

For information contact:	Brandon Young
Telephone:	561-228-2751
Email:	byoung@scripps.edu

Fee-for-Service Pricing, Effective October, 2010
(Subject to change, call for current pricing and quotes)

Qiagen Druggable Genome siRNA Library (v.2)

28,000 siRNAs generated against 7,000 target genes plated in 384 well format as a single siRNA per well at a concentration of 20 nM (1x 20 nM) or 4 pooled siRNA per well at a final concentration of 50 nM (12.5 nM for each siRNA) or 20 nM (5 nM for each siRNA).

1X 20 nM (88 plate set)	in white solid plates
	in black clear bottom
Pooled 50 nM (20 plate set)	in white solid plates
	in black clear bottom plates
Pooled 20 nM (20 plate set)	in white solid plates
	in black clear bottom plates

Mammalian Genome Collection (MGC) cDNA Library

16,953 human (6609 clones; 5537 unique sequences) and mouse (10,344 clones; 7718 unique sequences) cDNA sequences in an expressible vector are plated in 384 well format as 1 clone per well at a concentration of 40 ng.

MG C cDNA (50 plate set)	in white solid plates
	in black clear bottom plates

Hitpick and secondary screening of cDNA Library hits

Cost per individual experiment.

MGC focus set generation and screening: focus sets from the MGC cDNA library can be picked, prepped and plated in 96 or 384 well format then screened in a functional assay. Cost per individual experiment.

Gal4-Transcription Factor Library: 837 human or 721 mouse transcription factors fused to the Gal4

DNA binding domain plated in 384 well format as 1 clone per well at a concentration of 40 ng.

hGAL4-TFX (3 plate set)	in white solid plates
mGAL4-TFX  (2 plate set)	in white solid plates

Sigma Lopac

Library of Pharmacologically Active Compounds (1280 compounds)

Screening

The CBS core can run the screen for the investigator after the investigator has optimized the functional assay on a fee for service basis. We can also provide lipid reagents for transfection and read-out reagents such as BriteLite and AlphaScreen beads for screens run in-house at a bulk-price rate.

Screening Parameters:

Cell line: transfectability of cell line is paramount and requires optimization of lipid reagent used
Controls: Positive and negative controls are highly encouraged. Plate sets have space for 4 controls with an n=4 each (16 wells). Need minimal %CV.
Read-out:
1. Fluorescence
2. Luminescence
3. Absorbance
4. Alphascreen (homogenous bead based ELISA assay-PE)
Acceptable dynamic range (signal to noise)

***Pricing subject to change

Cell Based Screening Home