Scripps Florida

Cell Based Screening Core

















 

Access to Technologies

Cell Based Screening Core
External Pricing – Effective January 2008


For information contact: Juliana Conkright, Ph.D.
Telephone: 561-799-8950
Email: juliejc@scripps.edu

Qiagen Druggable Genome siRNA Library (v.2): 28,000 siRNAs generated against 7,000 target genes plated in 384 well format as a single siRNA per well at a concentration of 20 nM (1x 20 nM) or 4 pooled siRNA per well at a final concentration of 50 nM (12.5 nM for each siRNA) or 20 nM (5 nM for each siRNA).

1X 20 nM
(88 plate set)		 in white solid plates = $2550.00
in black clear bottom plates = $2900.00
Pooled 50 nM
(20 plate set)		 in white solid plates = $1450.00
in black clear bottom plates = $1525.00
Pooled 20 nM
(20 plate set)		 in white solid plates = $625.00
in black clear bottom plates = $700.00


Mammalian Genome Collection (MGC) cDNA Library: 16,953 human (6609 clones; 5537 unique sequences) and mouse (10,344 clones; 7718 unique sequences) cDNA sequences in an expressible vector are plated in 384 well format as 1 clone per well at a concentration of 40 ng.

MG C cDNA
(50 plate set)
in white solid plates=$1950.00
in black clear bottom plates=$2150.00


Hitpick and secondary screening of cDNA Library hits: cost per individual experiment.

MGC focus set generation and screening: focus sets from the MGC cDNA library can be picked, prepped and plated in 96 or 384 well format then screened in a functional assay. Cost per individual experiment

Gal4-Transcription Factor Library: 837 human or 721 mouse transcription factors fused to the Gal4

DNA binding domain plated in 384 well format as 1 clone per well at a concentration of 40 ng.

hGAL4-TFX
(3 plate set)		 in white solid plates=$85.00
mGAL4-TFX
(2 plate set)		 in white solid plates=$70.00


Sigma Lopac: Library of Pharmacologically Active Compounds (1280 cmpds)

LOPAC: $65.00 per set (4 plates) addition at 10 M concentration

Mutagenesis Screening: To identify residues important for gene function, the gene cDNA is chemically mutagenized. Clones are plated in 384 well format and screened in a functional assay. Hits can then be picked and counter-screened. Cost per individual experiment.

Screening

The CBS core can run the screen for the investigator after the investigator has optimized the functional assay on a fee for service basis. We can also provide lipid reagents for transfection and read-out reagents such as BriteLite and AlphaScreen beads for screens run in-house at a bulk-price rate.

Screening Parameters:

  1. Cell line: transfectability of cell line is paramount and requires optimization of lipid reagent used
  2. Controls: Positive and negative controls are highly encouraged. Plate sets have space for 4 controls with an n=4 each (16 wells). Need minimal %CV.
  3. Read-out:
    1. Fluorescence
    2. Luminescence
    3. Absorbance
    4. Alphascreen (homogenous bead based ELISA assay-PE)
  4. Acceptable dynamic range (signal to noise)


***Pricing subject to change