Scripps Research Joint Appointments

Translational Research Institute

Research Focus

My research interests lie in the development and application of mass spectrometry and proteomics techniques to the characterization of biochemical processes and pathways that play important roles in human health.   In particular, I am focused upon

• The development and application of HDX mass spectrometry for the characterization of protein dynamics
• Determination of protein posttranslational modifications with tandem mass spectrometry
• The development of methods for peptide ion fragmentation and their application to proteomics
• The application of quantitative proteomics techniques

Education

Ph.D., Chemistry, University of Manchester Institute of Science and Technology, 2001

Professional Experience

Post Doctoral Research, National High Magnetic Field Laboratory, Tallahassee, FL, USA, 2001-2004

Selected References

Choi, J.H.; Banks, A.D.; Estall, J.L.; Kajimura, S.; Laznik, D.; Mootha, V.; Chalmers, M.J.;   Kamenecka, T.M.; Griffin, P.R.; Spiegelman, B.M.  Obesity-linked phosphorylation of PPARg by cdk5 is a direct target of the anti-diabetic PPARγ ligands.  Nature.  2010. 466, 7305, 451-455

Pascal, B.D.; Chalmers, M.J.; Busby, S.A.; Griffin, P.; HD Desktop: an integrated platform for the analysis and visualization of H/D exchange data, Journal of the American Society for Mass Spectrometry, 2009, 20, 601-610.

Dai, S.; Chalmers, M.J.; Bruning, J.; Bramlett, K.S.; Osborne, H.E.; Montrose-Rafizadeh, C.; Barr, R.J.; Wang, Y.; Wang, M.; Burris, T.P.; Dodge, J.A.; Griffin, P.R. Prediction of the Tissue-Specificity of Selective Estrogen Receptor Modulators using a Single Biochemical Method. Proceedings of the National Academy of Sciences, 2008, 105(20): 7171-7176.

Bruning J, Chalmers M.J.; Prasad S.; Busby S.A.; Kamenecka T.; He Y.; Nettles K.W.; and Griffin P.R. Crystallography and H/D exchange Reveal that Graded Transcriptional Responses Mediated by PPAR are Controlled by Structural Dynamics, Structure, 2007, 15(10):1258-71.

Chalmers, M.J.; Busby, S.A.; Pascal, B.D.; He, Y.; Hendrickson, C.L.; Marshall, A.G.; Griffin, P.G.  Probing protein ligand interactions by automated hydrogen/deuterium exchange mass spectrometry.  Analytical Chemistry.  2006.  78, 1005-1014.

Chalmers, M.J.; Mackay, C.L.; Hendrickson, C.L.; Wittke, S.; Walden, M.; Mischak, H.; Fisler, D.; Just, I.; Marshall, A.G. Combined top-down and bottom-up mass spectrometric approach to characterization of biomarkers for renal disease. Analytical Chemistry. 2005. 77(22); 7163-7171.

Chalmers, M.J.; Håkansson, K.; Shen, J.; Smith, R.; Johnson, R.; Emmett, M.R.; Marshall, A.G. Protein kinase A phosphorylation characterized by tandem Fourier transform ion cyclotron resonance mass spectrometry. Proteomics, 2004. 4 (4) 970-981.

Links

Scripps Florida Mass Spectrometry and Proteomics