|
|
 |
Florida Faculty and Professional Staff
Peter Hodder
Scientific Director, Sr.
Director, Lead Identification
Translational Research Institute
TSRI - 2005
Joint Appointments Associate Professor, Molecular Therapeutics
Education
B.S., Chemistry, University of California, San Diego, 1994
Ph.D., Chemistry, University of Washington, 1999
Awards & Activities
Member of Society for Biomolecular Sciences
Association for Laboratory Automation.
Research Focus
High throughput screening (HTS) methodology employs sophisticated automation, detection & assay technologies to test large (several hundred thousand member) compound collections for biological or biochemical activity. It is a useful technique to identify "lead" compounds that will drive drug discovery research programs forward to a clinical drug candidate.
Our department operates TSRI's centralized HTS laboratory and manages its 600,000 member small-molecule HTS compound collection. This is accomplished by two state-of-the-art robotic platforms. One platform has the ability to test over one million assay wells per day in 1536-well plates, and stores the entire TSRI compound collection "on-line". The other is capable of instantly retrieving & reformatting any member of the collection to a 384 or 1536 well plate. Additionally, the department has ancillary laboratory equipment & expertise to implement HTS assays and facilitate downstream lead discovery efforts.
One of our major research goals is to collaborate with investigators who wish to test activity of the HTS compound collection against an assay of interest. Our typical collaborators comprise of TSRI faculty, Florida academicians, and scientists funded through the NIH's Molecular Library Screening Center Network (ttp://nihroadmap.nih.gov/). Another major research goal is to research & develop novel automation, instrumentation and assay technologies that benefit the HTS discipline.
o date, the Scripps Molecular Screening Center (SRIMSC) has completed more than 110 HTS campaigns and dose-response assays, and tested more than 4.6 million substances for the benefit of the scientific community. To find out more about the SRIMSC and its contributions to the MLSCN, please follow this link: http://molscreen.florida.scripps.edu/
Selected References
Hale, J.J., Lynch, C.L., Neway, W., Mills, S.G., Hajdu, R., Keohane, C.A., Rosenbach, M.J., Milligan, J.A., Shei,, G.J., Parent, S.A., Chrebet, G., Bergstrom, J., Card, D., Ferrer, M., Hodder, P., Strulovici, B., Rosen, H. and Mandala, S. A Rational Utilization of High-Throughput Screening Affords Selective, Orally Bioavailable 1-Benzyl-3-carboxyazetidine Sphingosine-1-phosphate-1 Receptor Agonists. Journal of Medicinal Chemistry, 2004, 47, 6662-6665.
Hodder, P.S., Mull, R., Cassaday, J., Berry, K., Strulovici, B. Miniaturization of Intracellular Calcium Functional Assays to 1536-Well Plate Format Using a Fluorimetric Imaging Plate Reader. Journal of Biomolecular Screening, 2004, 9(5), 417-426.
Kunapuli, P., Ransom, R., Murphy, K.L., Pettibone, D., Kerby, J., Grimwood, S., Zuck, P., Hodder, P., Lacson, R., Hoffman, I., Inglese, J., Strulovici, B. Development of an Intact Cell Reporter Gene Beta-Lactamase Assay for G Protein-Coupled Receptors for High-Throughput Screening, Analytical Biochemistry, 2003, 314 (1), 16-29.
Hodder P.S., Cassaday, J., Peltier, R., Berry, K., Inglese, J., Feuston, B., Culberson C., Bleicher L., Cosford N.D.P., Bayly C., Suto, C., Varney, M., Strulovici, B. Identification of Metabotropic Glutamate Receptor Antagonists Using an Automated High Throughput Screening System, Analytical Biochemistry, 2003, 313(2), 246-54.
|
|
|
|
