 |
Florida Faculty and Professional Staff
Donny Strosberg
Professor
Department of Infectology
TSRI - 2005
Joint Appointments Research Professor, Florida Atlantic University
Courtesy Professor, University of Florida
Education
1966 - 1970 Doctorate in Science (Chemistry)
Free University of Brussels (Belgium)
1962 - 1966 License in Chemistry,
Free University of Brussels (Belgium)
Awards & Activities
Member, EMBO, American Association of Immunologists
Associate Editor, Current Opinion in Biotechnology, Biologicals, Clinical Proteomics, Targets, Genetic Engineering News
Research Focus
Protein-protein interactions in host pathogen relations
Our laboratory analyzes the molecular determinants involved in protein-protein interactions in host pathogen relations. We currently focus on Hepatitis C viral infections of hepatoma cells or hepatocytes maintained in culture. Recombinant HCV protein domains that interact with each other have been produced in bacteria and purified by affinity chromatography. ELISA, Homogenous Time Resolved Fluorescence (“HTRF”) and Alpha screen assays have been developed to measure protein interactions and inhibition by peptides and small molecules. Peptides have thus been shown to block dimerization of a 106 N-terminal region of the HCV core protein. The same inhibitor peptides have been shown to block the release of HCV from infected host cells. Future work will aim at understanding differences linked to the viral genotypes, as was already observed for interferon alpha and cyclosporin A.
Selected References
Kota, S., Coito, C., Strosberg, A.D. “A Peptide Inhibitor of the Hepatitis C Virus Core Protein Dimerization” Proceedings of the 2nd International Workshop on Hepatitis C Resistance & New Compounds, Boston, MA. (2007)
Coito, C., Kota, S., Willoughby, D., Tellinghuisen, T.T., Strosberg, A.D. “Productions of Infectious Genotype 1b Hepatitis C Virus in human hepatoma cells.” Hepatology, Abstract # 483, Vol. 46, No. 4, Suppl. 1, P453A (2007)
Di Benedetto M, Bieche I, Deshayes F, Vacher S, Nouet S, Collura V, Seitz I, Louis S, Pineau P, Amsellem-Ouazana D, Couraud PO, Strosberg, AD, Stoppa-Lyonnet D, Lidereau R, Nahmias C. “Structural organization and expression of human MTUS1, a candidate 8p22 tumor suppressor gene encoding a family of angiotensin II AT2 receptor-interacting proteins, ATIP”. Gene 380, (2), 127-36 (2006).
Strosberg A.D. "Breaking the spell: drug discovery based on modulating protein-protein interactions" Expert Review of Proteomics, 1 (2), 141-143 (2004).
|
|
