Photo Gallery

Photo Gallery

	Figure 1: A spreading Fak -/- cell stained for focal adhesions with anti-vinculin (yellow-green) and for actin with rhodamine phalloidin (red). See Ren et al, 2000 J. Cell Sci.

Figure 2:
Smooth muscle cells stained with rhodamine phalloidin to label actin. The cell on the right was plated onto a prestretched elastic surface that was subsequently relaxed. The cell on the left is on the same matrix and then stretched. The cell in the center is the unmanipulated control. See Katsumi et al, 2002, submitted.

Figure 3:
NIH 3T3 fibroblasts injected with V12Rac and Alexa-PBD and FRET calculated as described in Kraynov et al 2000 to assess Rac interaction with PBD. Cells were treated with 5 uM beads coated with fibronectin. FRET positive zones appear at ruffling edges and around fibronectin coated beads. The corresponding phase images show the location on the beads in each cell.
See Del Pozo et al, 2002, Nature Cell Biol.

Figure 4:
Endothelial cells plated on silicone rubber surface that they can wrinkle. Inhibiting PAK by addition of a membrane permeant peptide induces a distinct loss of contractility over time. Images taken at 15 minute intervals. For comparison to the last image of the relaxed cell is a topographic map of California (final image in series). See Kiosses et al, 2002, submitted.

Figure 5:
Spreading FAK +/+ cell (top) and FAK -/- cells (bottom) stained for focal adhesions with anti-vinculin (yellow-green) and for actin stress fibers with rhodamine phalloidin (red). See Ren et al, 2000, J. Cell Sci.

Figure 6:
Endothelial cells containing GFP-Rac and Alexa 568-PBD were analyzed for Rac activation using the FRET assay. Cells were under static conditions or 10 dynes of shear stress (right). FRET positive edges (green-red) are predominantly on the downstream end of the cells. Images on top are of WT Rac-GFP (black and white).

Figure 7:
NIH 3T3 fibroblasts expressing a novel cdc42 exchange factor, Zizimin, that stimulates excessive filopodial formation in cells. Cells are labeled with rhodamine phalloidin (left) and on the right are complimentary pairs of barrel cacti for comparison. See Meller et al, submitted.

Figure 8:
An endothelial cell expressing dominant negative PAK (green), stained for focal adhesions with anti-vinculin (yellow-green) and for actin stress fibers with rhodamine phalloidin (red). See Kiosses et al, J. Cell Biol. 1999.

Figure 9:
An endothelial cell triple labeled (on the right) with rhodamine phalloidin (red), anti-Beta 3 (blue) and wow-1 (green), and also showing a ratio image (darker green) and the USA. On the left is the merged triple labeled confocal image on the cell. See Kiosses et al, 2001, Nature Cell Biol.