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Next Generation Sequencing Core

Technology

The TSRI Genomics Core is a full-service facility providing library preparation and sequencing services. The facility currently operates Illumina and Oxford Nanopore sequencing systems, as well as a Fluidigm, Biomark HD, and Bio-Rad digital droplet PCR system.

Our Illumina NextSeq 500 system typically generates 75 - 150 base single-end reads or up to 2 X 150 paired ends reads with outputs of about 400 million reads per run. Run times are typically 1 - 2 days. The Illumina Miseq sequencing system is used for 2 X 75 base or 2 X 300 base reads, generating up to 25 million reads per run. The Genomics core also operates a Oxford Nanopore long read sequencing system

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The facility is also equipped with a 10X Genomics droplet instrument that allows for single-cell gene expression or imunne profiling applications, as well as, other applications.

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Our Bio-Rad QX200 digital droplet PCR system can be used for a variety of assay such as;

  • Cancer biomarker studies and copy number variation 
  • Pathogen detection .
  • Next generation sequencing 
  • Gene expression analysis 

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The BioMark™ HD System performs high throughput real-time PCR and digital PCR, integrating thermal cycling and fluorescence detection. 96 samples can be tested with 96 PCR primer sets generating 9,216 qPCR data points in a single run. Protocols for single-cell qPCR have been successfully implemented on our system. Using the Fluidigm Access Array, one can PCR amplify 48 different samples with up to 48 different PCR primer sets and recover the products for Next Generation Sequencing analysis.