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Next Generation Sequencing Core

Applications

Next Generation Sequencing is a versatile technology with many genomic applications. This technology is readily adapted to addressing unique sequencing problems on a high throughput scale. Both single (sequencing from one end of library fragments) and paired end (sequencing from both ends of library fragments) reads of up to 150 bases can be generated from most libraries. Library are typically generated with 100-500 base inserts and require between 10 ng and 1 ug of sample DNA or RNA. Methods for Mate-pair sequencing (sequencing fragments separated by defined spacing of 1-10kb) are also available. Some common applications are described below:

  1. Identification of polymorphisms and chromosomal rearrangements, sequencing novel genomes Libraries are prepared from fragmented genomic DNA and sequenced using single-read or paired-end reads of up to 100 bases in length. Data can be compared to a reference genome or assembled de novo for novel genomes.
  2. ChIP seq Libraries are prepared from Immunoprecipitated DNA or RNA are sequenced and aligned to a reference genome.
  3. RNA seq Libraries are prepared from RNA derived from any source. Methods are available that preserve strand orientation of the original RNA source. Methods for making libraries from nanogram quantities of RNA are routinely used in our lab.
  4. Epigenetics, PCR products, library sequencing etc. Please contact our lab to discuss specific sequencing applications.