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Nikon Spinning Disk Confocal

Nikon Spinning Disk Confocal Microscope with Perfect Focus (Room MBB37)


The Nikon Spinning Disk Confocal is optimized for time-lapse confocal imaging of fluorescent probes in live cells, with minimal photo bleaching and higher time resolution compared to the single-point laser scanning confocal systems

Nikon Spinning Disk Confocal

Hourly Rate for Usage: TSRI trained user hourly rates $10.00 from 9AM-5PM Monday-Friday, $8 from 6-9AM, 5-9PM and during weekends. $50 flat rate overnight.
Contact kpestonj@scripps.edu for training.
 

The TE2000 microscope with perfect focus and automated XYZ stage, is equipped with a Yokagawa CSU22 spinning disk head with the Borealis system from Spectral Applied Research, including a new high power 4-line laser launch (405, 488 561, and 642 laser lines).


The microscope is attached to the following infinity corrected optics:

  • 10X 0.3 NA PlanFluor Ph1 DLL
  • 20X 0.5 NA Plan Fluor Ph1 DLL
  • 40X 1.0 NA Oil Plan Apo Ph3 DM
  • 60X 1.4 NA Oil Pan Apo Ph3 DM
  • 100X 1.4 NA Oil Plan Apo Ph3 DM

In addition to the standard 4 color (DAPI/FITC/Red/Cy5) channels, the CSU22 also has filters that would allow CFP/YFP imaging and FRET (by the ratiometric method) using the 405 laser for CFP excitation. The microscope is equipped with an incubator for live cell imaging.  Images are acquired using a CoolSnap HQ2 camera with the Metamorph acquisition and analysis software.