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Microscopy - Deconvolution

OlympusDeltaVision Optical Sectioning Microscope Model 283: This system consists of an Olympus IX-70 microscope, a mercury arc lamp excitation source, a high precision piezoelectric motorized XYZ stage and a wide array of objective lenses. 

  • 100X oil, UPlanApo-1.35na
  • 60X oil, PlanApo-1.4na
  • 40X oil, UApo-1.35na
  • 40X air, UPlanApo-0.85na
  • 40X air, UPlanFlu-0.60na., Phase 2
  • 20X air, PlanApo,0.50na, Phase 1
  • 10X air Plan Apo, 0.4na, Phase 1

Scientific ImageThis deconvolution microscope is equipped for viewing live specimens with a Live Cell 3 temperature, humidity and CO2 controlled live chamber (Pathology Devices, MD) and a Bioptechs Objective heater (Biotechs, Inc, PA). Images are acquired on a Photometrics CH350L liquid cooled CCD camera (500KHz, 12bit, 2MP, KAF1400GI, 1317x1035), which collects especially low intensity signals with an linear response to light intensity (CCD Quantum efficiency: Dapi:37%, FITC:50%, Rhodamine, Texas Red:63%, Cy5:63%). The acquired images are subsequently deconvolved using DeltaVision software softWoRx (API Inc.) or Autoquant (Media Cybernetics) on the Agard / Sadat inverse matrix algorithm. The fluorescence filter sets currently installed permit the use of DAPI, FITC, GFP, PE, Rhodamine, Texas Red, Cy5, CFP, YFP as well as DIC. The System is ideal for fluorescence and phase cell tracking assays, auto focus, point-revisiting as well as long term live fluorescence acquisition.

Scientific Images Image

Workstation 1 An additional workstation (DECON1) is also available with the Softworks (API Inc.) analysis software, as well as Image J (vers 3.4), GIMP and Movie Maker (UNIX equivalent of Adobe Premier) for image analysis. Raw Delta vision images can also be imported and analyzed using Imaris (Bitplane Inc.) or Autoquant (Media Cybernetics) software with full retention of the original data (on Conf 5 or 8).
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