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RSA 2006 Abstracts
Oregon Health & Science University
TRANSCRIPTOME META-ANALYSIS FILTER BY CONGENIC DATASET IDENTIFIES CANDIDATE GENES FOR ALCOHOL PREFERENCE QTL: S. E. Bergeson; M. K. Mulligan; H. M. Kamens; T. J. Phillips. Waggoner Center for Alcohol and Addiction Research, University of Texas, Austin, TX 78712, Department of Behavioral Neuroscience, Oregon Health & Science University, Portland, OR 97239.
Construction of congenic strains of mice capturing QTL has been useful for fine mapping and narrowing the region associated with a given phenotype. A congenic mouse strain for a large QTL on chromosome 9 was created with a DBA/2J (D2) region from 9-61 cM introgressed onto a C57BL/6J (B6) background. Results of two-bottle choice alcohol drinking showed that mice from the progenitor background strain drank 12.5 g/kg/day while the B6.D2Ch9 drank significantly less (9.6 g/kg/day, N=38-40). Although interval specific crosses will provide a more narrow focus, we chose to first use the congenic strain as a novel reagent for identifying cis-regulation on chromosome 9 and trans-regulation by chromosome 9. Using cDNA microarray analysis of both progenitor B6 and B6.D2Ch9 mice jointly housed and age-matched, a dataset of expression divergence became a filter for a larger meta-analysis for alcohol preference drinking. Overlap analysis revealed sixteen known genes and four ESTs as candidate QTG. Supported by NIAAA.
UNPREDICTABLE CHRONIC MILD STRESS AND VOLUNTARY ETHANOL CONSUMPTION IN INBRED MICE: E. H. Beckley; D. A. Finn. Portland Alcohol Research Center, Portland Veterans Affairs Medical Center Research, Portland, OR 97239 & Department of Behavioral Neuroscience, Oregon Health & Science University, Portland, OR 97239.
Evidence suggests that alcoholism is linked to psychological disturbances such as depression, but the unpredictable chronic mild stress (UCMS) model of depression has not been widely explored as a method to study voluntary ethanol intake in laboratory rodents. Therefore, we adapted a UCMS procedure using singly housed male FVB/NJ mice housed on a reversed light/dark cycle (lights on from 1700 to 0500). Control group (“No Stress” n = 14) and stress group (two groups of n = 15) mice were presented with a 10% v/v solution of ethanol for two 4 hr periods during their dark cycle (0700 to 1100 “AM”, and 1230 to 1630 “PM”), every other day. After establishing baseline intake (2 wks), a 4-wk manipulation phase commenced. During the manipulation phase, stressed group mice were subjected to unpredictable, mild stressors (9 different stress conditions) either during their ethanol drinking period (“Day On” group) or on the day that they were not presented with ethanol (“Day Off” group), whereas the control group remained undisturbed in their home cages. After the manipulation phase all mice were tested in the Forced Swim Test (FST) to assess differences in depression-like behavior between treatment groups. Drinking increased as a main effect of time during the 4-wk
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