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Center for Protein and Nucleic Acid Research

Custom Peptide Arrays on Glass Slides

This service is run as either a collaborative full-service - where we will assist in your assays - or as a stand alone service - where we just synthesize the arrays and run control assays to ensure synthesis integrity. However, some training may be necessary for scanning and processing the slides.

Call 4-9941 for pricing and estimated delivery date.

Prep 5 image

This instrument is capable of synthesizing up to 1536 different peptides in about 1-2 weeks. Peptides are synthesized on a 2 mm sized nitrocellulose disk. Each disk is then dissolved in 0.5 mL of solvent, 60 nL of which is spotted onto specially coated microscope slides and allowed to dry. Once dry, the spots will not wash off in aqueous solution.
Comparing peptide arrays on glass slides to arrays on nitrocellulose membranes, glass slides offer:

  1. Ability to make hundreds of arrays from one array synthesis (the most expensive part), whereas nitrocellulose membranes are typically only used for a few experiments.
  2. Glass slides allow for smaller volumes for binding/activity assays, 3-4 mL for standard incubations, and as little as 120 uL for diffusion-controlled cover slip assays.
  3. Peptide spots on glass slides are 3-dimensional structures that contain up to 100 times more peptide per area than conventional monlayer deposition (see figure below).
Polimer Bound Peptides Peptitde Array
Phosphorylation
Sitemapping