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Center for Protein and Nucleic Acid Research

Custom Peptide Libraries and Arrays on Glass Slides

This service is run as either a collaborative full-service - where we will assist in your assays - or as a stand alone service - where we just synthesize the peptides and/or arrays and run control assays to ensure synthesis integrity. Some training may be necessary for scanning and processing slides.

Please call or email to discuss potential projects. (858) 784-9941 (4-9941, internal)  philo@scripps.edu or rmcbride@scripps.edu

Prep 5 image

This instrument is capable of synthesizing up to 1536 different peptides in each synthesis. Peptides are synthesized on a nitrocellulose disk. Each peptide is then cleaved from the nitrocellulose membrane, resulting in about 35 ug of each peptide. The peptides can be used directly or they can be arrayed on a microscope slide by employing orthogonal coupling chemistry and specially modified hydrogel microscope slides.
Comparing peptide arrays on glass slides to arrays on nitrocellulose membranes, glass slides offer:

  1. Ability to make hundreds of arrays from one array synthesis, whereas nitrocellulose membranes are typically only used for a few experiments.
  2. Glass slides allow for smaller volumes for binding/activity assays, 3-4 mL for standard incubations, and as little as 120 uL for diffusion-controlled cover slip assays.
  3. Peptide spots on hydrogel glass slides are 3-dimensional structures that contain up to 100 times more peptide per area than conventional monlayer deposition (see figure below).
Polimer Bound Peptides Peptitde Array
Phosphorylation
Sitemapping