This form can be used in two different ways. You can either enter your
sequence (lower/upper case, spaces OK)
or enter the number of each nucleotide (in addition to the absorbance,
sample volume of stock measured, total volume in cuvette*, and total
If you choose to enter the number of each nucleotide, be sure that the
empty (the 'Clear Form' button will ensure this). Also, make sure to choose
whether your oligo is DNA (the default) or RNA.
*Defaults have been set for these values, but they can be changed.
Concentrations are based on the sum of the extiction coefficients for the monomers, NOT on nearest neighbor method.