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Center for Protein and Nucleic Acid Research

Attention: This is our external web site. If you are attempting to place an order through our on-line ordering system you will have to access our web site on the intranet

Standard Sanger Sequencing

The center uses the ABI 3730 DNA analyzer for standard sanger sequencing. Reactions are performed using thermal cycle sequencing conditions (annealing at 50 C) with fluorescently labeled terminators. Turnaround time for data is approximately one business day. Questions, email dnaseq@scripps.edu.

DNA Sequencing Order Form

DNA alignment software - Sequencher and Primer design software- Oligo 6, are both available for use in our office at MB 101.

Tips, Downloads, Policies, & Protocols

Submission requirements and first time users

Primer sequence information

Seq. failure policies and meaning of DNA comments

Freeware for PC's and Mac's to analyze data

How to download your data from our website

DNA troubleshooting

Sequencing of G-C rich/secondary structure templates

Microcon DNA clean-up procedure

Recommended DNA template purification protocols

Description of different data file types