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DNA Array Core Facility

RMA Express

RMAExpress is a standalone GUI program for Windows (and Linux) to compute gene expression summary values for Affymetrix Genechip® data using the Robust Multichip Average expression summary. It does not require R nor is it dependent on any component of the Bioconductor project.

http://stat-www.berkeley.edu/users/bolstad/RMAExpress/RMAExpress.html

Features:

  • Point-and-click simplicity of a GUI
  • Perfect Match (PM) only model
  • Model-based expression intensity generation
  • Quantile normalization

Input:

Affymetrix .CEL files and .CDF file for the chip type

Output:

.CSV file of signal intensities based on the Affymetrix .CEL file

Availability:

RMAExpress is available for download through the RMAExpress website