Issue 3 (February 15, 1997) Volume 23 of Blood Cells, Molecules, & Diseases (ISSN 1079-9796)
French, D.L., Coller, B.S. - Hematologically Important Mutations: Glanzmann Thrombasthenia . . . . . 39-51

Reprint requests to: Deborah L. French, Ph.D., Mount Sinai School of Medicine, Box 1079, Division of Hematology, One Gustave L. Levy Place, New York, NY 10029 USA, phone: (212) 241-8286, fax: (212) 996-1029, e-mail: dfrench@smtplink.mssm.edu.
View the PDF file

Issue 3 (February 15, 1997) Volume 23 of Blood Cells, Molecules, & Diseases (ISSN 1079-9796)
Jagadeeswaran, P., Liu, Y.C. - A Hemophilia Model in Zebrafish: Analysis of Hemostasis . . . . . 52-57

ABSTRACT. We have developed artificial hemophilia in zebrafish by treating them with copper and measured their clotting function by a newly developed sensitive clotting time assay. The clotting function can be detected rapidly and reliably in 30 hr larvae and in adult fish by measuring the blood clotting time. We have used this assay to screen wild type zebrafish and identified fish with prolonged clotting time. This verifies the usefulness of this assay in future screening for recessive hemostasis defects generated by chemical and radiation mutagenesis methods.

Keywords: hemophilia, zebrafish, hemostasis, clotting time, copper, mutagenesis.

Reprint requests to: Dr. Pudur Jagadeeswaran, Department of Cellular and Structural Biology, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, Texas USA 78284-7964, phone: (210) 567-3834, fax: (210) 567-3803, e-mail: jagadeeswar@uthscsa.edu.
View the PDF file

Issue 3 (February 15, 1997) Volume 23 of Blood Cells, Molecules, & Diseases (ISSN 1079-9796)
Krantz, A. - Red Cell-Mediated Therapy: Opportunities and Challenges . . . . . 58-68

ABSTRACT. Red blood cells have unique properties that offer attractive therapeutic possibilities. It is proposed that by covalently linking drug pharmacophores to red cell surface proteins, significant enhancement of pharmacokinetics can be achieved. Specific anchoring to erythrocytes can be accomplished in principle, using unique, affinity labeling, combinatorial libraries targeting the surface protein, glycophorin A. The anticipated advantages of red cell-anchored drugs over free drugs, include extended half-lives, controlled volumes of distribution, and multivalent interactions.

Keywords: covalent labeling, affinity labels, bonding, combinatorial libraries, anchors.

Reprint requests to: Allen Krantz, Ph.D., Red Cell Inc., 270-B Littlefield Avenue, South San Francisco, CA 94080, phone: (415) 827-6000, fax: (415) 8277-0123, email: allen@redcell.com.
View the PDF file